Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis
This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer,...
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Published in | Chinese science bulletin Vol. 53; no. 19; pp. 2953 - 2957 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Heidelberg
SP Science in China Press
01.10.2008
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Subjects | |
Online Access | Get full text |
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Summary: | This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes. |
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Bibliography: | Q51 11-1785/N capillary sieving electrophoresis, semi-crosslinked polyacrylamide, protein separation |
ISSN: | 1001-6538 2095-9273 1861-9541 2095-9281 |
DOI: | 10.1007/s11434-008-0430-2 |