Semi-crosslinked polyacrylamides as high-resolution and dynamic self-coating sieving matrices for protein capillary electrophoresis

• Published in: Chinese science bulletin Vol. 53; no. 19; pp. 2953 - 2957
• Main Authors: Zhou, Jin, Xu, JianDong, Xie, Yao, Qu, Feng, Deng, YuLin, Geng, LiNa
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science in China Press 01.10.2008
• Subjects: Chemistry/Food Science; Earth Sciences; Engineering; Humanities and Social Sciences; Life Sciences; multidisciplinary; Physics; Science; Science (multidisciplinary); 毛细管筛子; 聚丙烯酰胺; 蛋白质分离; protein separation; semi-crosslinked polyacrylamide; capillary sieving electrophoresis
• ISSN: 1001-6538; 2095-9273; 1861-9541; 2095-9281
• DOI: 10.1007/s11434-008-0430-2

This paper describes non-gel capillary sieving electrophoresis employing semi-crosslinked polyacrylamide as a high performance and low viscous replaceable separation matrix for separation of non-denatured protein separation. Arising from the fine sieving and dynamic coating ability of this polymer, a mixture of basic proteins lysozyme, cytochrome C, ribonuclease A, and trypsin was resolved with excellent reproducibility. Mixing different semi-crosslinked polyacrylamides together further improves the separation. The separtion mechanism was analyzed. With network structure developed to an intermediate state between crosslinked gel and linear polymer solutions, these semi-crosslinked polyacrylamide polymers demonstrate a promise as a new class of size sieving separation medium, not only in capillary electrophoresis, but also in microfluidic chip separation schemes.