Correlations between the structure and anti-diabetic activity of novel polysaccharides from raw and “Nine Steaming Nine Sun-Drying” Polygonti rhizome

“Nine Steaming Nine Sun-Drying” Polygonti rhizome has been used as valuable tonic health-care products for thousands of years. This research aimed to determine the correlations between the structure and anti-diabetic activities of three novel polysaccharides isolated from the raw and “Nine Steaming...

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Published inInternational journal of biological macromolecules Vol. 260; no. Pt 2; p. 129171
Main Authors Yu, Jinqian, Zhao, Lei, Wang, Zhenqiang, Yue, Tao, Wang, Xiao, Liu, Wei
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.03.2024
Subjects
Animals
Anti-diabetic activity
Glucose - analysis
Mice
Plant Extracts - chemistry
Polygonti rhizome
Polysaccharides
Polysaccharides - chemistry
Rhizome - chemistry
Steam
PBS
BCA
DMSO
1D and 2D NMR
IR
Mw
Akt
MTT
HPAEC
AUC
DMEM
SD
GSK3β
Polysaccharides
Anti-diabetic activity
ANOVA
Polygonti rhizome
OGTT
HE
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Summary:“Nine Steaming Nine Sun-Drying” Polygonti rhizome has been used as valuable tonic health-care products for thousands of years. This research aimed to determine the correlations between the structure and anti-diabetic activities of three novel polysaccharides isolated from the raw and “Nine Steaming Nine Sun-Drying” Polygonti rhizome, with PRP-R from the raw ones and PRP-9Z and PRP-9A from the steamed ones. Structures of the isolated polysaccharides were determined by IR and NMR spectra, as well as monosaccharide composition and methylation analysis. In vitro assays indicated that PRP-9Z could improve the glucose consumptions more effectively than PRP-R and PRP-9A via Akt/GSK3β insulin signal pathway by western blotting analysis. In vivo assays indicated PRP-9Z could improve the glucose tolerance in the BKS-db mice. Histopathological assay demonstrated that PRP-9Z effectively reduced the damage of the kidney and liver. The above results indicated that PRP-9Z from “Nine Steaming Nine Sun-Drying” Polygonti rhizome showed significant anti-diabetic properties, which indicated that PRP-9Z with higher content of →1)-β-Frup-(2→ was more active than PRP-R with higher →1,6)-β-Fruf-(2→ and PRP-9A with higher →4)-β-Galp(1→.
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ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.129171