CRISPR-Cas12a trans -cleaves DNA G-quadruplexes

We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing...

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Published inChemical communications (Cambridge, England) Vol. 56; no. 83; pp. 12526 - 12529
Main Authors Li, Ying, Li, Tao, Liu, Bi-Feng, Hu, Rui, Zhu, Jiang, He, Ting, Zhou, Xin, Li, Conggang, Yang, Yunhuang, Liu, Maili
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 20.10.2020
Subjects
Aptamers, Peptide - metabolism
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
Circular Dichroism
CRISPR
CRISPR-Associated Proteins - chemistry
CRISPR-Associated Proteins - metabolism
CRISPR-Cas Systems - genetics
DNA Cleavage
Electrophoresis
Endodeoxyribonucleases - chemistry
Endodeoxyribonucleases - metabolism
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
G-Quadruplexes
Humans
Kinetics
NMR
Nuclear magnetic resonance
RNA, Guide, CRISPR-Cas Systems - metabolism
Telomere - metabolism
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Summary:We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.
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ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc05540a