Establishment and validation of a 2D primary gill cell culture of the sevenband grouper (Hyporthodus septemfasciatus)

A 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell lay...

Full description

Saved in:
Bibliographic Details
Published inJournal of virological methods Vol. 327; p. 114922
Main Authors Rajendran, Rahul, Krishnan, Rahul, Oh, Myung-Joo
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.2024
Subjects
2D primary cell culture
Animals
Bass - virology
Cell Culture Techniques - methods
Cells, Cultured
Fish Diseases - virology
Gill epithelium
Gills - cytology
Gills - virology
Host-Pathogen Interactions
NNV
Nodaviridae - physiology
Primary Cell Culture - methods
RNA Virus Infections - veterinary
RNA Virus Infections - virology
Sevenband grouper
Virus Replication
Sevenband grouper
2D primary cell culture
Gill epithelium
NNV
Online AccessGet full text

Cover

More Information
Summary:A 2D primary gill cell culture system of the sevenband grouper (Hyporthodus septemfasciatus) was established to validate the pathogenesis of nervous necrosis virus (NNV) as observed in previous studies. This system, developed using the double-seeded insert (DSI) technique, yielded confluent cell layers. Upon challenge with NNV in a setup containing both autoclaved salt water and L15 media in the apical compartment, viral replication akin to that anticipated based on previous studies was observed. Consequently, we advocate for the utilization of primary gill cell culture as a viable alternative to conventional methodologies for investigating host pathogen interactions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Undefined-3
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2024.114922