Ontogenic development of autoantibody repertoires in spleen and peritoneal cavity of normal mice: examples of T cell-dependent and -independent reactivities

The ontogenic development of B cell clonal precursors (BCP) reactive to bromelain-treated, syngeneic erythrocytes (BrMRC) and to single-stranded DNA has been studied by limiting dilution of both spleen and peritoneal cells. It was found that the frequency of anti-BrMRC BCP in the spleen is very low...

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Bibliographic Details
Published inEuropean journal of immunology Vol. 19; no. 7; p. 1195
Main Authors Huetz, F, Poncet, P, Coutinho, A, Portnoï, D
Format Journal Article
LanguageEnglish
Published Germany 01.07.1989
Subjects
Age Factors
Animals
Antigens, T-Independent - immunology
Autoantibodies - immunology
B-Lymphocytes - immunology
DNA, Single-Stranded - immunology
Erythrocytes - immunology
Mice
Mice, Inbred Strains
Mice, Nude
Peritoneal Cavity - immunology
Rats
Spleen - immunology
T-Lymphocytes - immunology
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Summary:The ontogenic development of B cell clonal precursors (BCP) reactive to bromelain-treated, syngeneic erythrocytes (BrMRC) and to single-stranded DNA has been studied by limiting dilution of both spleen and peritoneal cells. It was found that the frequency of anti-BrMRC BCP in the spleen is very low up to 4 weeks of age and slowly increases thereafter, to reach adult levels by 6-10 weeks. In the peritoneal cavity, no such BCP can be found before 2 weeks, but they occur at a very high frequency already by 3 weeks of age. Injection of adult, normal syngeneic T cells at birth has no apparent effect on the representation of anti-BrMRC BCP in the peritoneal cavity, but brings these to adult levels or even higher in the spleen already at 3 weeks of age. Accordingly, adult athymic (nude) mice contain normal frequencies of BrMRC-specific BCP in the peritoneal cavity but are devoid of such clones in the spleen. In contrast, the frequency of anti-DNA BCP is very high throughout postnatal development in both spleen and peritoneal cavity, of normal and athymic mice, in both resting and naturally activated splenic B cell compartments, and it is independent of T cell transfers into nude animals. These results indicate the role of T cells in the establishment of some clonal specificities in the adult, splenic autoreactive B cell repertoire.
ISSN:0014-2980
DOI:10.1002/eji.1830190707