The First Cholera Case Diagnosed Early in the Clinical Laboratory by DNA Probe Method

An alkaline-phosphatase-labelled synthetic oligonucleotide probe was applied to detect the structural gene of cholera enterotoxin (ctx). This DNA probe has a complementary base sequence to 30 base of the CT-A subunit. This method was, for the first time, applied to diagnosis of a diarrheal patient....

Full description

Saved in:
Bibliographic Details
Published inKansenshogaku Zasshi Vol. 66; no. 12; pp. 1645 - 1650
Main Authors MIYAGI, Kazufumi, OMURA, Kanzo, NODA, Koji, YOSHIDA, Akio, MORI, Hideto, SUZUKI, Norihiko, TAKAI, Shinya, MATSUMOTO, Yasukazu, HAYASHI, Kazu, YOH, Myonsun, YAMAMOTO, Koichiro, HONDA, Takeshi
Format Journal Article
LanguageJapanese
English
Published Japan The Japanese Association for Infectious Diseases 01.12.1992
Subjects
Adult
alkaline-phosphatase-labelled probe
Cholera - diagnosis
Cholera - epidemiology
cholera enterotoxin
DNA probe method
DNA Probes
Enterotoxins - analysis
Enterotoxins - genetics
Feces - microbiology
Humans
Japan - epidemiology
Male
Travel
Japan
Online AccessGet full text

Cover

More Information
Summary:An alkaline-phosphatase-labelled synthetic oligonucleotide probe was applied to detect the structural gene of cholera enterotoxin (ctx). This DNA probe has a complementary base sequence to 30 base of the CT-A subunit. This method was, for the first time, applied to diagnosis of a diarrheal patient. The probe detected ctx rapidly and simply as compared with reversed passive latex agglutination (RPLA) and Beads-ELISA. The cfu minimal dose for detection with the probe was about 106-7/ml. This method can be easily performed in any clinical laboratory because the procedure is safe, simple and rapid (it can be completed within about 3 hours).
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Report-3
ObjectType-Case Study-4
ISSN:0387-5911
1884-569X
DOI:10.11150/kansenshogakuzasshi1970.66.1645