Cdc2 and Cdk2 Kinase Activated by Transforming Growth Factor-β1 Trigger Apoptosis through the Phosphorylation of Retinoblastoma Protein in FaO Hepatoma Cells
The signaling pathway leading to TGF-β1-induced apoptosis was investigated using a TGF-β1-sensitive hepatoma cell line, FaO. Cell cycle analysis demonstrated that the accumulation of apoptotic cells was preceded by a progressive decrease of the cell population in the G1 phase concomitant with a slig...
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Published in | The Journal of biological chemistry Vol. 274; no. 45; pp. 31775 - 31783 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Inc
05.11.1999
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Online Access | Get full text |
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Summary: | The signaling pathway leading to TGF-β1-induced apoptosis was investigated using a TGF-β1-sensitive hepatoma cell line, FaO. Cell cycle analysis demonstrated that the accumulation of apoptotic cells was preceded by a progressive decrease of the cell population in the G1 phase concomitant with a slight increase of the cell population in the G2/M phase in response to TGF-β1. TGF-β1 induced a transient increase in the expression of Cdc2, cyclin A, cyclin B, and cyclin D1 at an early phase of apoptosis. During TGF-β1-induced apoptosis, the transient increase in cyclin-dependent kinase (Cdk) activities coincides with a dramatic increase in the hyperphosphorylated forms of RB. Treatment with roscovitine or olomoucine, inhibitors of Cdc2 and Cdk2, blocked TGF-β1-induced apoptosis by inhibiting RB phosphorylation. Overexpression of Bcl-2 or adenovirus E1B 19K suppressed TGF-β1-induced apoptosis by blocking the induction of Cdc2 mRNA and the subsequent activation of Cdc2 kinase, whereas activation of Cdk2 was not affected, suggesting that Cdc2 plays a more critical role in TGF-β1-induced apoptosis. In conclusion, we present the evidence that Cdc2 and Cdk2 kinase activity transiently induced by TGF-β1 phosphorylates RB as a physiological target in FaO cells and that RB hyperphosphorylation may trigger abrupt cell cycle progression, leading to irreversible cell death. |
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ISSN: | 0021-9258 1083-351X |
DOI: | 10.1074/jbc.274.45.31775 |