Cell cycle distribution defect in PHA-stimulated T lymphocytes of sickle cell disease patients

T lymphocytes from normal human controls and sickle cell disease (SCD) patients were isolated from peripheral blood and cultured for 72 hours following addition of phytohemagglutinin. The ratio for the fraction of cells in DNA synthesis (S phase) over the fraction in G2 phase (S/G2) was significantl...

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Bibliographic Details
Published inAmerican journal of hematology Vol. 28; no. 4; p. 279
Main Authors Abdallah, J M, Kukuruga, M, Nakeff, A, Prasad, A S
Format Journal Article
LanguageEnglish
Published United States 01.08.1988
Subjects
Adolescent
Adult
Anemia, Sickle Cell - immunology
Anemia, Sickle Cell - pathology
Cell Cycle - drug effects
Humans
Interphase
Lymphocyte Activation
Male
T-Lymphocytes - immunology
Zinc - pharmacology
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Summary:T lymphocytes from normal human controls and sickle cell disease (SCD) patients were isolated from peripheral blood and cultured for 72 hours following addition of phytohemagglutinin. The ratio for the fraction of cells in DNA synthesis (S phase) over the fraction in G2 phase (S/G2) was significantly higher in SCD patients in comparison to the controls (mean +/- SD) (4.01 +/- 0.78 vs. 2.78 +/- 0.76, P less than 0.02). Following in vivo zinc supplementation to two subjects, the S/G2 ratio was normalized. We conclude that the distribution of T lymphocytes in cell cycle is altered in SCD patients and that this effect may be zinc-dependent.
ISSN:0361-8609
DOI:10.1002/ajh.2830280414