Evaluation of the antibacterial efficacy of nanomodified triple antibiotic paste with nano anti-inflammatory drug and nano calcium hydroxide as intracanal medicament using confocal laser scanning microscope

• Published in: Endodontology : journal of Indian Endodontic Society Vol. 36; no. 1; pp. 25 - 29
• Main Authors: Prakhyath, Alekhya, Parvathaneni, Krishna, Raju, T. G, Seshadri, Abitha, Kintada, Venkata, Parepalli, Prem
• Format: Journal Article
• Language: English
• Published: Medknow Publications and Media Pvt. Ltd 01.01.2024; Wolters Kluwer Medknow Publications
• Subjects: active pharmaceutical ingredient; Amoxicillin; anti-inflammatory; Hydroxides; Ibuprofen; Metronidazole; nano calcium hydroxide; nano triple antibiotic paste; Root canal therapy; India
• ISSN: 0970-7212; 2543-0831
• DOI: 10.4103/endo.endo_34_23

Aim: To evaluate and compare the antibacterial efficacy of Nano amoxicillin modified Triple antibiotic paste (mTAP) with nano Ibuprofen and nano calcium hydroxide (Ca(OH)2) as a root canal medicament on Enterococcus faecalis infected root canal dentin.. Methods: Sixty eight semi-cylindrical root samples were infected for 3 weeks with E. faecalis ATCC 29212 and randomly assigned into 4 Groups based on the medication used, Ca(OH)2; Nano Ca(OH)2; Amoxicillin mTAP +I (Ibuprofen); Nano Amoxicillin mTAP + Nano Ibuprofen and was incubated for 7 days. The proportions of dead and live bacteria was quantitatively analyzed by Confocal laser scanning microscopy. One-way ANOVA was used to compare the four groups and intergroup comparison with Tukey’s post hoc tests. Results: Nano Amoxicillin mTAP with Nano Ibuprofen was shown to have a higher antibacterial effect against E. faecalis compared to the unmodified medicaments. Conclusion: Nanomodified ICMs of APIs showed better antimicrobial activity due to their increased stability and deeper penetration into dentinal tubules when compared to conventional forms of ICMs.