Identification of polyoma BK virus in kidney transplant recipients by shell vial cell culture assay and urine cytology

• Published in: American journal of clinical pathology Vol. 126; no. 3; pp. 444 - 447
• Main Authors: SEMPLE, Kenrick, LOVCHIK, Judith, DRACHENBERG, Cinthia
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Society of Clinical Pathologists 01.09.2006
• Subjects: Antigens, Viral, Tumor - immunology; Biological and medical sciences; BK virus; BK Virus - isolation & purification; Cells, Cultured; Cost-Benefit Analysis; Humans; Investigative techniques, diagnostic techniques (general aspects); Kidney Transplantation; Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase Chain Reaction; Sensitivity and Specificity; Urine - cytology; Cell culture; Shell vial cell culture; Polyomavirus; Cytology; Urine culture; Identification; BK virus; Papovaviridae; Homotransplantation; Kidney; Infection; Virus; Basic science; Anatomic pathology; Shell; Urinary system; Viral disease
• ISSN: 0002-9173; 1943-7722
• DOI: 10.1309/KMNJAX3BH4DG5FC0

Urine cytology often is used to identify BK virus in kidney transplant recipients in the cytology laboratory. To assess the usefulness of the shell vial cell culture assay to identify BK virus, urine samples from 42 kidney transplant recipients were tested by the urine cytology and shell vial cell culture assays. The shell vial cell culture assay is just as sensitive and specific as urine cytology for the identification of BK virus in kidney transplant recipients.