A porphyrin-triazatruxene dyad for ratiometric two-photon fluorescent sensing of intracellular viscosity
By combining an electron-rich triazatruxene unit ( TAT ) to an electron-deficient zinc porphyrin fluorophore ( ZnPor ) via an ethynyl bridge, a new two-photon fluorescent viscosity rotor ( TAT-ZnPor ) with typical donor-π-acceptor (D--A) electronic configuration was developed for the ratiometric two...
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Published in | Journal of materials chemistry. B, Materials for biology and medicine Vol. 1; no. 28; pp. 5487 - 5492 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Cambridge
Royal Society of Chemistry
20.07.2022
|
Subjects | |
Online Access | Get full text |
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Summary: | By combining an electron-rich triazatruxene unit (
TAT
) to an electron-deficient zinc porphyrin fluorophore (
ZnPor
)
via
an ethynyl bridge, a new two-photon fluorescent viscosity rotor (
TAT-ZnPor
) with typical donor-π-acceptor (D--A) electronic configuration was developed for the ratiometric two-photon fluorescent detection of intracellular viscosity. The
TAT-ZnPor
dyad exhibited highly improved fluorescence quantum yield (
Φ
em
= 0.40) and two-photon absorption cross-section (
δ
TPA
= 811 GM) in comparison to the individual components. In the methanol/glycerol system,
TAT-ZnPor
showed sensitive fluorescence responses toward the change of viscosity. Upon elevating the viscosity from 0.59 to 947 cp, the blue emission band around 410 nm gradually enhanced, while the red band at 647 nm concomitantly quenched, leading to a remarkable intensity ratio (
I
410
/
I
647
) change from 0.70 to 81 (116-fold).
TAT-ZnPor
also displayed good cell imaging performance under one- and two-photon excitation, and strong mitochondria targeting ability in living cells, thus was successfully applied in detecting the change of mitochondrial viscosity during the nystatin-induced degeneration.
A new fluorescent viscosity rotor (
TAT-ZnPor
) shows highly improved two-photon action cross section and sensitive ratiometric response to mitochondrial viscosity in living cells. |
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Bibliography: | https://doi.org/10.1039/d2tb00384h Electronic supplementary information (ESI) available. See DOI ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2050-750X 2050-7518 |
DOI: | 10.1039/d2tb00384h |