The effect of dissolved oxygen partial pressure on the growth and carbohydrate metabolism of mouse LS cells

Batch cultures of mouse LS cells were grown in suspension at controlled dissolved oxygen partial pressures (pO2). At low pO2 (1·6 mmHg) the growth and respiration rates and the final cell population were all limited. At high pO2 (320 mmHg), cell division was inhibited after an initial doubling of th...

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Bibliographic Details
Published inJournal of cell science Vol. 4; no. 1; pp. 25 - 37
Main Authors Kilburn, D G, Lilly, M D, Self, D A, Webb, F C
Format Journal Article
LanguageEnglish
Published England 01.01.1969
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Summary:Batch cultures of mouse LS cells were grown in suspension at controlled dissolved oxygen partial pressures (pO2). At low pO2 (1·6 mmHg) the growth and respiration rates and the final cell population were all limited. At high pO2 (320 mmHg), cell division was inhibited after an initial doubling of the cell number. At intermediate values of pO2, the growth rate was constant but the final cell population varied. Within the pO2 range of 40 –100 mmHg, the final cell population was constant and maximal at 1·2 × 106 viable cells/ml. Except at 320 mmHg pO2, about 90 % of the glucose consumed served as an energy source and could be accounted for as lactate and CO,. In the culture at 320 mmHg, only 60 % of the glucose consumed could be accounted for in this way. During growth the production of lactate and pyruvate was highest at low pO2. A sharp increase in lactate production was observed as logarithmic growth ceased in each culture, except at high pO2 (160 mmHg). These observations indicate that pO2 markedly influences cell growth and carbohydrate metabolism in these cells.
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content type line 23
ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.4.1.25