Simple Method for the Determination of Cefaclor in Human Plasma Samples by HPLC

• Published in: Journal of liquid chromatography & related technologies Vol. 26; no. 19; pp. 3315 - 3323
• Main Authors: Granados-Soto, V., Aguilar-Cota, M. E., Reyes-Garcia, G., Medina-Santillán, R., Flores-Murrieta, F. J.
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 01.11.2003; Taylor & Francis
• Subjects: Analysis; Biological and medical sciences; Cefaclor; General pharmacology; HPLC; Human plasma; Medical sciences; Pharmacokinetics; Pharmacology. Drug treatments; Human; Biological fluid; Cephalosporin derivatives; Chemical analysis; β-Lactams; Oral administration; HPLC chromatography; Bioavailability; Antibacterial agent; Pharmacokinetics; Cefaclor; Blood plasma
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-120025526

A rapid and simple method for determination of cefaclor in human plasma samples, by high-performance liquid chromatography (HPLC), was developed. This method includes a deproteinization with perchloric acid as extraction procedure. Plasma extracts were analyzed on a reversed phase column eluted with a mixture of acetonitrile and 0.01 M sodium dihydrogen phosphate solution, adjusted to pH 6.5 with potassium hydroxide (7:93, v/v), and detected by absorbance at 260 nm. Retention times for cefaclor and the internal standard were 5.2 and 7.2 min, respectively. The method was linear in the range of 0.5-30 µg/mL and the detection limit of the method was 100 ng/mL. This method was useful and suitable for determination of cefaclor after oral administration in healthy volunteers, and could be used for bioavailability and bioequivalence studies of the drug.