Induction of two alcohol dehydrogenase polypeptides in rice roots during anaerobiosis

• Published in: Plant science (Limerick) Vol. 54; no. 1; pp. 29 - 36
• Main Authors: Kadowaki, Koh-Ichi, Matsuoka, Makoto, Murai, Norimoto, Harada, Kyuya
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1988; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; alcohol dehydrogenase; anaerobiosis; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; induction; Metabolism; Metabolism. Physicochemical requirements; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Plant physiology and development; rice; root; induction; root; alcohol dehydrogenase; anaerobiosis; rice; ADH, alcohol dehydrogenase; DTT, dithiothreitol; Proteins; Monocotyledones; Anoxia; Gramineae; Enzyme; Angiospermae; Spermatophyta; Alcohol dehydrogenase; Cereal crop
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/0168-9452(88)90052-0

In vivo pulse labeling of rice roots exposed to anaerobiosis shows that new proteins are synthesized while the synthesis of other proteins ceases as a result of the stress. Alcohol dehydrogenase (ADH) activity in the rice roots gradually increased over a 24-h period, leveling off subsequently. An antiserum against ADH was used to show that ADH is present at low levels in aerobically grown roots, and that two molecular forms of ADH, differing in molecular weight, are synthesized de novo when the roots are stressed. The rise in ADH activity under a lack of oxygen does not involve the conversion of an inactive protein into active enzyme.