High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions

High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 58; no. 2; p. 221
Main Authors Farinha, M A, Kropinski, A M
Format Journal Article
LanguageEnglish
Published England 01.07.1990
Subjects
DNA, Bacterial - pharmacology
Electrochemistry
Freezing
Plasmids
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - genetics
Transformation, Bacterial
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Summary:High efficiency transformation of Pseudomonas aeruginosa was achieved using frozen cell suspensions and high voltage electroporation. We have obtained frequencies as high as 5.8 x 10(8) transformants/micrograms of plasmid DNA using PA01 strain OT684 and a buffer of 15% glycerol-1 mM MOPS. The method allows for easy and reproducible production of frozen cell suspensions for rapid transformation of P. aeruginosa.
ISSN:0378-1097
DOI:10.1016/S0378-1097(05)80043-6