Tungsten disulfide Quantum Dots Based Disposable Paper Based Lab on GenoChip for Specific Meningitis DNA Detection

• Published in: Journal of the Electrochemical Society Vol. 167; no. 10; pp. 107501 - 107508
• Main Authors: Gupta, Riya, Ozhukil Valappil, Manila, Sakthivel, Arunkumar, Mathur, Ashish, Pundir, Chandra Shekhar, Murugavel, Kathiresan, Narang, Jagriti, Alwarappan, Subbiah
• Format: Journal Article
• Language: English
• Published: IOP Publishing 28.05.2020
• Subjects: Bacterial Meningitits; DNA Sensors; Electrochemical Genosensor; Paper-based analytical device (PAD); Tungsten disulfide quantum dots (WS2QDs)
• ISSN: 0013-4651; 1945-7111; 1945-7111
• DOI: 10.1149/1945-7111/ab8fda

Tungsten disulfide quantum dots (WS2 QDs) embedded lab on genochip based analytical device (PAD) was developed for the specific DNA detection of Meningitis, a life threatening disease. This paper-based lab on genochip was designed by grafting the two electrodes onto the surface of paper using conductive ink. The WS2 QDs were characterized by X-ray diffraction technique, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Following this, specific oligonucleotide sequence of DNA (capture probe) was immobilized over the surface of WS2 QDs/PAD electrode. The ssDNA/WS2QDs/PAD device was characterized electrochemically by cyclic voltammetry. The device was employed for detection of target DNA (by hybridization) by employing methylene blue (MB) as an indicator. The biosensor exhibited a wide linear response in the range 1 nM-100 M towards the target DNA. The limit of detection (LOD) of target DNA of the proposed device is 1 nM. The advantageous features of this device than the existing biosensors include cheaper analysis cost, tiny sample requirements and stability.