2-(5-Hydrazinocarbonyl-2-thienyl) -5, 6-methylenedioxybenzofuran and 2-(5-hydrazinocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran as novel fluorescence derivatisation reagents for carboxylic acids in liquid chromatography

• Published in: Analytica chimica acta Vol. 300; no. 1; pp. 243 - 251
• Main Authors: Saito, Mikihiko, Ushijima, Tamano, Sasamoto, Kazumi, Yakata, Kenshi, Ohkura, Yosuke, Ueno, Keiyu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.01.1995; Elsevier
• Subjects: Acid hydrazides; Analytical chemistry; Carboxylic acids; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Fluorescence derivatisation reagents; Fluorimetry; Furyl benzofuran; Liquid chromatography; Other chromatographic methods; Prostaglandins; Thiophenyl benzofuran; Prostaglandins; Furyl benzofuran; Fluorescence derivatisation reagents; Acid hydrazides; Liquid chromatography; Carboxylic acids; Fluorimetry; Thiophenyl benzofuran; Organic hydrazide; Chemical analysis; Chemical reagent; Multicomponent mixture; Precolumn; Tricyclic compound; Derivatization; Experimental study; Oxygen heterocycle; Fluorescence spectrometry; Sulfur heterocycle; Carboxylic acid; Detection limit; Preparation
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/0003-2670(94)00422-I

The new acid hydrazides, 2-(5-hydrazinocarbonyl-2-thienyl)-5,6-methylenedioxybenzofuran (TMBH) and 2-(5-hydrazin-ocarbonyl-2-furyl)-5, 6-methylenedioxybenzofuran (FMBH), were synthesized as precolumn fluorescence derivatisation reagents for carboxylic acids in liquid chromatography. These compounds readily react with carboxylic acids in the presence of a coupling reagent under mild conditions in aqueous solution to give fluorescent derivatives. The detection limits of lauric acid were both 0.1 pmol per 10 μl injection volume at a signal-to-noise ratio of 3. The methods in which these compounds are used were applied to the assay of a standard mixture of prostaglandins (25 μM) and prostaglandins in a human seminal fluid.