Protocol for calcium imaging and analysis of hippocampal CA1 activity evoked by non-spatial stimuli

The hippocampus has a major role in processing spatial information but has been found to encode non-spatial information from multisensory modalities in recent studies. Here, we present a protocol for recording non-spatial stimuli (visual, auditory, and a combination) that evoked calcium activity of...

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Published inSTAR protocols Vol. 5; no. 2; p. 103110
Main Authors Sun, Dechuan, Amiri, Mona, Unnithan, Ranjith Rajasekharan, French, Chris
Format Journal Article
LanguageEnglish
Published Elsevier Inc 21.06.2024
Elsevier
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microscopy
neuroscience
Protocol
single cell
single cell
neuroscience
microscopy
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Summary:The hippocampus has a major role in processing spatial information but has been found to encode non-spatial information from multisensory modalities in recent studies. Here, we present a protocol for recording non-spatial stimuli (visual, auditory, and a combination) that evoked calcium activity of hippocampal CA1 neuronal ensembles in C57BL/6 mice using a miniaturized fluorescence microscope. We describe steps for experimental apparatus setup, surgical procedures, software development, and neuronal population activity analysis. For complete details on the use and execution of this protocol, please refer to Sun et al.1 [Display omitted] •Instructions for measuring non-spatial stimuli that evoked hippocampal CA1 activity•Steps for calcium imaging in the mouse hippocampal CA1 region•Guidance on calcium imaging data analysis pipelines•Procedures for neuronal population activity analysis Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. The hippocampus has a major role in processing spatial information but has been found to encode non-spatial information from multisensory modalities in recent studies. Here, we present a protocol for recording non-spatial stimuli (visual, auditory, and a combination) that evoked calcium activity of hippocampal CA1 neuronal ensembles in C57BL/6 mice using a miniaturized fluorescence microscope. We describe steps for experimental apparatus setup, surgical procedures, software development, and neuronal population activity analysis.
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ISSN:2666-1667
2666-1667
DOI:10.1016/j.xpro.2024.103110