Analysis of rabbit cell surface (CD) antigens by means of cross-reactive monoclonal antibodies with specificity for cattle CD antigens

• Published in: Czech Journal of Animal Science Vol. 54; no. 6; pp. 270 - 276
• Main Authors: Simon, M.,Slovenska Akademia Vied, Ivanka pri Dunaji (Slovak Republic). Ustav Biochemie a Genetiky Zivocichov, Antalikova, J.,Slovenska Akademia Vied, Ivanka pri Dunaji (Slovak Republic). Ustav Biochemie a Genetiky Zivocichov, Horovska, L.,Slovenska Akademia Vied, Ivanka pri Dunaji (Slovak Republic). Ustav Biochemie a Genetiky Zivocichov, Jankovicova, J.,Slovenska Akademia Vied, Ivanka pri Dunaji (Slovak Republic). Ustav Biochemie a Genetiky Zivocichov, Fabryova, K.,Slovenska Akademia Vied, Ivanka pri Dunaji (Slovak Republic). Ustav Biochemie a Genetiky Zivocichov, Hluchy, S.,Slovenska Polnohospodarska Univ., Nitra (Slovak Republic), Chrenek, P.,Slovenska Polnohospodarska Univ., Nitra (Slovak Republic), Tancin, V.,Slovenska Polnohospodarska Univ., Nitra (Slovak Republic)
• Format: Journal Article
• Language: English
• Published: Czech Academy of Agricultural Sciences 01.06.2009
• Subjects: ANIMAL TISSUES; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; ANTIGENS; BLOOD CELLS; BOVIN; CATTLE; cd41/61; cd9; CELLULE SANGUINE; CELULAS SANGUINEAS; CONEJO (ORYCTOLAGUS); ELISA; GANADO BOVINO; http://www.fao.org/aos/agrovoc#c_1391; http://www.fao.org/aos/agrovoc#c_15968; http://www.fao.org/aos/agrovoc#c_24051; http://www.fao.org/aos/agrovoc#c_27963; http://www.fao.org/aos/agrovoc#c_32817; http://www.fao.org/aos/agrovoc#c_3804; http://www.fao.org/aos/agrovoc#c_3807; http://www.fao.org/aos/agrovoc#c_4298; http://www.fao.org/aos/agrovoc#c_504; http://www.fao.org/aos/agrovoc#c_6414; http://www.fao.org/aos/agrovoc#c_960; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; IMMUNOLOGICAL TECHNIQUES; INMUNOFLUORESCENCIA; LAPIN (ORYCTOLAGUS); LEUCOCITOS; LEUCOCYTE; LEUKOCYTES; MONOCLONAL ANTIBODIES; RABBITS; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS; TEJIDOS ANIMALES; TEST ELISA; TISSU ANIMAL; tissue
• ISSN: 1212-1819; 1805-9309
• DOI: 10.17221/1728-cjas

Studies that involved testing monoclonal antibodies (mAbs) for cross-species reactivity proved to be efficient for the identification of previously unrecognized antigens in a number of different species. Twenty-six mAbs specific to different bovine CD (cluster defined) antigens (CD9, CD18, CD45R, CD41/61, CD62L, MHC class I and bovine IgG light chain molecule) were assayed for reactivity with rabbit peripheral blood leukocytes. Four of the mAbs recognizing CD9 and CD41/61 were reactive with rabbit platelets or granulocytes. These were investigated further by immunoblotting and immunohistochemical staining. The study identified CD9 and CD41/61 molecules on rabbit cells by mAbs IVA-50 and IVA-38. It showed that IVA-50 is a new valuable CD9 reagent for rabbit immunology which could be used for immunofluorescence staining or ELISA assay, immunohistological and molecular studies of rabbit CD9 antigen. IVA-38 recognizes the CD41/61 on rabbit platelets in indirect immunofluorescence and ELISA assay.