Amyloid aggregation and secondary structure changes of liver cystatin: Acidic denaturation and TFE induced studies

• Published in: Journal of biomolecular structure & dynamics Vol. 40; no. 23; pp. 12506 - 12515
• Main Authors: Mir, Faisal Mustafa, Bano, Bilqees
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2022
• Subjects: aggregation; amyloid; Amyloid - chemistry; CD spectroscopy; Circular Dichroism; Cystatin; Cystatins - chemistry; FTIR; Liver; Protein Structure, Secondary; Solvents; Cystatin; aggregation; amyloid; FTIR; CD spectroscopy

A cysteine proteinase inhibitor has been purified by affinity chromatography from the liver of buffalo. Liver cystatin is subjected to incubation at low pH with co-solvent TFE, where we have studied the effect on the conformation, activity and tendency to form aggregates or fibrils. ANS fluorescence...