Microsatellites for the at-risk Mottled Duskywing butterfly, Erynnis martialis

• Published in: Conservation genetics resources Vol. 14; no. 4; pp. 387 - 388
• Main Authors: Kroeze, Shayla L., Brewster, Adrienne, Linton, Jessica E., Norris, D. Ryan, Otis, Gard W., Young, Yvonne, Keyghobadi, Nusha
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2022; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; Conservation Biology/Ecology; Ecology; Erynnis martialis; Evolutionary Biology; Funding; Genetics; Heterozygosity; Life Sciences; Methods and Resources Article; Microsatellites; Plant Genetics and Genomics; Population differentiation; Population genetics; Reintroduction; Software; Species at risk; Microsatellite markers; Next generation sequencing; Reintroduction; Conservation genetics
• ISSN: 1877-7260; 1877-7252; 1877-7260
• DOI: 10.1007/s12686-022-01280-5

We describe 24 microsatellite loci for an at-risk North American butterfly, the Mottled Duskywing ( Erynnis martialis ). Forty-one perfect tetra and di-nucleotide loci were originally identified using Illumina sequencing and were refined to 24 variable and cleanly amplifiable loci, which we attempted to characterized in 34 individuals from a single population. We detected 2 to 10 alleles per locus. Observed heterozygosity ranged from 0.06 to 0.76, and expected heterozygosity ranged from 0.06 to 0.79. We also describe conditions by which 18 microsatellites can be multiplexed in five separate reactions. These microsatellites will be used to investigate population differentiation and diversity of Mottled Duskywing, to inform reintroduction and conservation protocols for the species in Ontario, Canada where it is listed as endangered.