First Gynogenesis of Vanilla planifolia for Haploid Production and Ploidy Verification Protocol

Vanilla orchids are members of the Vanilloideae orchid subfamily, and they hold significant economic value as a spice crop in tropical regions. Despite the presence of 180 known species within this subfamily, commercial production focuses on only three species ( , , and ) and one hybrid ( ), prized...

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Bibliographic Details
Published inPlants (Basel) Vol. 13; no. 13; p. 1733
Main Authors Gastelbondo, Manuel, Nicholls, Ursula, Chen, Sisi, Chambers, Alan, Wu, Xingbo
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 23.06.2024
Subjects
Aromatic compounds
Bioactive compounds
Chloroplasts
Chromosomes
Crop diseases
Crop production
Crops
Flow cytometry
Genetic analysis
Genetic improvement
Genomes
Germplasm
Gynogenesis
haploids
Haplotypes
Industrial development
Morphology
Ovaries
Oxidation
Plant breeding
Ploidy
R&D
Research & development
Seeds
Stomata
Tissue culture
Tropical environment
Tropical environments
Vanilla
Vanilla planifolia
gynogenesis
haploids
Vanilla planifolia
tissue culture
ploidy
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Summary:Vanilla orchids are members of the Vanilloideae orchid subfamily, and they hold significant economic value as a spice crop in tropical regions. Despite the presence of 180 known species within this subfamily, commercial production focuses on only three species ( , , and ) and one hybrid ( ), prized for their aromatic qualities and bioactive compounds. Limited modern breeding initiatives have been undertaken with vanilla orchids, although recent advancements in genomic research are shedding light on this crop's potential. The protracted breeding cycle of vanilla, coupled with increasing demand for germplasm, underscores the importance of research and breeding efforts in vanilla. This paper outlines a protocol for haploid production in using unfertilized ovaries in tissue culture conditions. Additionally, we present a methodology to confirm the haploid nature of putative haploid lines through stomatal size comparison, chromosome counting, and flow cytometry analysis, proving the successful development of haploid vanilla plants. These findings contribute to the advancement of breeding programs and genetic improvement strategies for the vanilla industry.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants13131733