Detection and characterization of incidental vibrations from Drosophila suzukii in infested fruits

• Published in: Journal of pest science Vol. 97; no. 3; pp. 1461 - 1473
• Main Authors: Fellin, Lorenzo, Bertagnolli, Giulia, Mazzoni, Valerio, Anfora, Gianfranco, Agostinelli, Claudio, Rossi Stacconi, Marco Valerio, Nieri, Rachele
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2024; Springer Nature B.V
• Subjects: Age; Agriculture; Amplitudes; Berries; Biomedical and Life Sciences; Blueberries; Damage detection; Drosophila suzukii; Eclosion; Ecology; Entomology; Forestry; Frequency ranges; Fruit flies; Fruits; Indigenous species; Infestation; Insects; Introduced species; Invasive species; Larvae; Larval development; Life Sciences; Original Paper; Pests; Plant Pathology; Plant Sciences; Predators; Ripening; Spectral analysis; Spectrum analysis; Statistical analysis; Substrate inhibition; Vaccinium; Vibration analysis; Vibration meters; Vibrations; Wooden structures; Biotremology; Non-destructive detection; Spotted-wing drosophila; Laser vibrometer
• ISSN: 1612-4758; 1612-4766
• DOI: 10.1007/s10340-023-01711-8

The spotted-wing drosophila (SWD), Drosophila suzukii (Matsumura), is an invasive species native to East Asia and now widespread worldwide. Major economic damage is caused by the larvae developing within ripening soft-skin fruit. Currently, larval detection in fruit is limited to destructive methods and post-harvest control strategies heavily rely on the use of chemicals or cold to inhibit egg eclosion and larval growth. Feeding larvae are likely to induce substrate-borne vibrations in the berry that could be exploited as cues by predators or to develop a non-invasive pest detection method, an approach previously applied on leaves and wooden structures, but never on fresh fruit. We used a laser vibrometer to detect and characterize the incidental vibrations produced by D. suzukii larvae within fresh blueberries at five different pest age (48, 96, 168, 216 and 264 h). An innovative statistical analysis was performed to assess if infestation level (number of pupae) and pest age (hours after exposure) affect the spectrum and the amplitude of vibrations. The recordings of infested berries were characterized by the presence of a series of broad-band pulses (frequency range 0.1–2 kHz) without a regular temporal pattern, in an amplitude range between 12.1 and 946 µm/s. Furthermore, the analysis revealed the possibility to distinguish between different pest ages and infestation levels. By a spectral analysis of the recordings, the pest ages can be distinguished among each other, but for the age groups at 168 and 216 h after infestation. The vibration amplitude trend gradually increased up to 168–216 h after infestation, and then decreased until fly emergence. Low-infested blueberries showed a faster D. suzukii development time compared to high-infested blueberries. This was reflected into vibrational recordings, as low-infested blueberries exhibited peak amplitude at earlier stage compared to high-infested ones. Results suggest that D. suzukii larvae induce detectable vibrations by feeding within berries that are dependent on infestation level and pest age. We discuss the possible ecological role of such vibrations as cues for unintended receivers, such as predators and parasitoids, and their potential for innovative infestation detection methods.