Fabricating a reactive surface on the fibroin film by a room-temperature plasma jet array for biomolecule immobilization

A simple dielectric barrier discharge（DBD） jet array was designed with a liquid electrode and helium gas.The characteristics of the jet array discharge and the preliminary polymerization with acrylic acid（AA） monomer were presented.The plasma reactor can produce a cold jet array with a gas temperatu...

Full description

Saved in:
Bibliographic Details
Published inChinese physics B Vol. 21; no. 10; pp. 313 - 320
Main Author 陈光良 郑旭 吕国华 张朝霞 Sylvain Massey Wilson Smith Michael Tatoulian 杨思泽
Format Journal Article
LanguageEnglish
Published 01.10.2012
Subjects
Acrylic acid
Arrays
Discharge
Fourier transforms
Infrared spectroscopy
Monomers
Plasma (physics)
Scanning electron microscopy
介质阻挡放电
傅立叶变换红外光谱
反应性
固定化
生物分子
等离子体射流
表面粗糙度
阵列
Online AccessGet full text
ISSN1674-1056
2058-3834
1741-4199
DOI10.1088/1674-1056/21/10/105201

Cover

More Information
Summary:A simple dielectric barrier discharge（DBD） jet array was designed with a liquid electrode and helium gas.The characteristics of the jet array discharge and the preliminary polymerization with acrylic acid（AA） monomer were presented.The plasma reactor can produce a cold jet array with a gas temperature lower than 315 K,using an applied discharge power between 6 W and 30 W（V dis × I dis）.A silk fibroin film（SFF） was modified using the jet array and AA monomer,and the treated SFF samples were characterized by atomic force microscopy（AFM）,scanning electron microscopy（SEM）,Fourier transform infrared spectroscopy（FTIR）,and contact angle（CA）.The deposition rate of the poly acrylic acid（PAA） was able to reach 300 nm/min,and the surface roughness and energy increased with the AA flow rate.The FTIR results indicate that the modified SFF had more carboxyl groups（-COOH） than the original SFF.This latter characteristic allowed the modified SFF to immobilize more quantities of antimicrobial peptide（AP,LL-37） which inhibited the Escherichia coli（E.Coli） effectively.
Bibliography:plasma jet array; polymerizing modification; fibroin film; antimicrobial property
A simple dielectric barrier discharge（DBD） jet array was designed with a liquid electrode and helium gas.The characteristics of the jet array discharge and the preliminary polymerization with acrylic acid（AA） monomer were presented.The plasma reactor can produce a cold jet array with a gas temperature lower than 315 K,using an applied discharge power between 6 W and 30 W（V dis × I dis）.A silk fibroin film（SFF） was modified using the jet array and AA monomer,and the treated SFF samples were characterized by atomic force microscopy（AFM）,scanning electron microscopy（SEM）,Fourier transform infrared spectroscopy（FTIR）,and contact angle（CA）.The deposition rate of the poly acrylic acid（PAA） was able to reach 300 nm/min,and the surface roughness and energy increased with the AA flow rate.The FTIR results indicate that the modified SFF had more carboxyl groups（-COOH） than the original SFF.This latter characteristic allowed the modified SFF to immobilize more quantities of antimicrobial peptide（AP,LL-37） which inhibited the Escherichia coli（E.Coli） effectively.
11-5639/O4
Chen Guang-Liang,Zheng Xu ,L Guo-Hua ,Zhang Zhao-Xia ,Sylvain Massey ,Wilson Smith ,Michael Tatoulian , Yang Si-Ze （1. Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education,and Engineering Research Center for Eco-Dyeing ＆ Finishing of Textiles,Zhejiang Sci-Tech University,Hangzhou 310018,China b） Key Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences,Beijing 100190,China c） ＇ Ecole Nationale Sup＇erieure de Chimie de Paris-Chimie ParisTech,Laboratoire de G＇enie des Proc＇ed＇es Plasmas et Traitements de Surfaces,Universit＇e Pierre et Marie Curie,Paris 75005,France
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1674-1056
2058-3834
1741-4199
DOI:10.1088/1674-1056/21/10/105201