Characterization of Urease Immobilized Plant Biomass and Its Application to Indirect Determination of Formaldehyde

Unreacted urea remaining after the reaction of urea with formaldehyde was converted to ammonia using immobilized urease. The quantity of ammonia produced was measured using IC, thus, the remaining formaldehyde was indirectly quantified. The immobilized urease was prepared by the adsorption of urease...

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Published inBUNSEKI KAGAKU Vol. 72; no. 10.11; pp. 449 - 454
Main Authors MIYAUCHI, Toshiyuki, IGAKI, Yui, MIURA, Koki, SHIKAMI, Yuko
Format Journal Article
LanguageEnglish
Japanese
Published The Japan Society for Analytical Chemistry 05.10.2023
Subjects
biomass
cedar
formaldehyde
IC-FIA
immobilized urease
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Summary:Unreacted urea remaining after the reaction of urea with formaldehyde was converted to ammonia using immobilized urease. The quantity of ammonia produced was measured using IC, thus, the remaining formaldehyde was indirectly quantified. The immobilized urease was prepared by the adsorption of urease on woody and herbaceous biomass modified with polyamine. The activity of the immobilized urease was the highest in cedar (53.8 U mg−1), followed in descending rice (40.4 U mg−1), kenaf (34.0 U mg−1), and beech (11.5 U mg−1). A fixed quantity of urea was reacted with formaldehyde in the presence of hydrochloric acid. Unreacted urea was passed through an IC-FIA system connected to the immobilized urease column and the ammonia produced from the urea was measured. The calibration curves of formaldehyde were linear (r = 0.9968) over 1.0-5.0 mg, therefore, formaldehyde could be quantified. The determination of formaldehyde was affected by acetaldehyde, but not by other aldehydes.
ISSN:0525-1931
DOI:10.2116/bunsekikagaku.72.449