Visualization of viral DNA assembly using nonfluorescent staining
We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilit...
Saved in:
Published in | Biotechnic & histochemistry Vol. 94; no. 5; pp. 309 - 312 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
England
Taylor & Francis
04.07.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilitates their use in everyday experiments. Methyl green is most promising for routine detection of viral DNA assemblies in the cytoplasm; the procedure enables ready detection of viral DNA accumulation in the cytoplasm. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1052-0295 1473-7760 |
DOI: | 10.1080/10520295.2018.1530453 |