Visualization of viral DNA assembly using nonfluorescent staining

We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilit...

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Published inBiotechnic & histochemistry Vol. 94; no. 5; pp. 309 - 312
Main Authors Karalova, E. M., Hakobyan, L. H., Tatoyan, M. R., Sahakyan, K. T., Karalyan, Z. A.
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 04.07.2019
Subjects
African Swine Fever Virus - isolation & purification
Animals
Azure Stains
Cytoplasm - virology
DNA
DNA, Viral - analysis
Macrophages, Alveolar - virology
Methyl Green
methyl green pyronin
nonfluorescent staining
Oxazines
Staining and Labeling - methods
Swine
viral assemblies
Virus Assembly
viral assemblies
nonfluorescent staining
methyl green pyronin
DNA
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Summary:We present an easy test for rapid visualization of viral DNA assemblies in infected cell cytoplasm. We selected the best stains for nuclear staining: Nile blue A, Bismarck brown, gallocyanin chrome alum, methyl green pyronin and azure II. None of the staining techniques is fluorescent, which facilitates their use in everyday experiments. Methyl green is most promising for routine detection of viral DNA assemblies in the cytoplasm; the procedure enables ready detection of viral DNA accumulation in the cytoplasm.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1052-0295
1473-7760
DOI:10.1080/10520295.2018.1530453