Shroom family proteins regulate gamma-tubulin distribution and microtubule architecture during epithelial cell shape change

Cell shape changes require the coordination of actin and microtubule cytoskeletons. The molecular mechanisms by which such coordination is achieved remain obscure, particularly in the context of epithelial cells within developing vertebrate embryos. We have identified a novel role for the actin-bind...

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Bibliographic Details
Published inDevelopment (Cambridge) Vol. 134; no. 7; pp. 1431 - 1441
Main Authors Lee, Chanjae, Scherr, Heather M, Wallingford, John B
Format Journal Article
LanguageEnglish
Published England 01.04.2007
Subjects
Actins - metabolism
Animals
Cell Shape - physiology
Cytoskeleton - physiology
Epithelial Cells - cytology
Image Processing, Computer-Assisted
Immunoblotting
Immunohistochemistry
In Situ Hybridization
Microfilament Proteins - metabolism
Microscopy, Confocal
Microtubules - metabolism
Tubulin - metabolism
Xenopus - embryology
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Summary:Cell shape changes require the coordination of actin and microtubule cytoskeletons. The molecular mechanisms by which such coordination is achieved remain obscure, particularly in the context of epithelial cells within developing vertebrate embryos. We have identified a novel role for the actin-binding protein Shroom3 as a regulator of the microtubule cytoskeleton during epithelial morphogenesis. We show that Shroom3 is sufficient and also necessary to induce a redistribution of the microtubule regulator gamma-tubulin. Moreover, this change in gamma-tubulin distribution underlies the assembly of aligned arrays of microtubules that drive apicobasal cell elongation. Finally, experiments with the related protein, Shroom1, demonstrate that gamma-tubulin regulation is a conserved feature of this protein family. Together, the data demonstrate that Shroom family proteins govern epithelial cell behaviors by coordinating the assembly of both microtubule and actin cytoskeletons.
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ISSN:0950-1991
1477-9129
DOI:10.1242/dev.02828