Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy

• Published in: Chemical communications (Cambridge, England) Vol. 59; no. 98; pp. 14563 - 14566
• Main Authors: Yamakoshi, Hiroyuki, Shibata, Daiki, Bando, Kazuki, Kajimoto, Shinji, Kohyama, Aki, Egoshi, Syusuke, Dodo, Kosuke, Iwabuchi, Yoshiharu, Sodeoka, Mikiko, Fujita, Katsumasa, Nakabayashi, Takakazu
• Format: Journal Article
• Language: English
• Published: CAMBRIDGE Royal Soc Chemistry 07.12.2023; Royal Society of Chemistry
• Subjects: Chemistry; Chemistry, Multidisciplinary; Glutathione; Microscopy; Physical Sciences; Raman spectroscopy; Science & Technology; THIOLS; PROBES

Ratiometric Raman analysis of reversible thia-Michael reactions was achieved using α-cyanoacrylic acid (αCNA) derivatives. Among αCNAs, the smallest derivative, ThioRas (molecular weight: 167 g mol −1 ), and its glutathione adduct were simultaneously detected in various subcellular locations using R...