N.m.r. lipid profiles of cells, tissues and body fluids : neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions
Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond...
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Published in | Biochemical journal Vol. 290; no. 3; pp. 717 - 721 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Colchester
Portland Press
15.03.1993
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Subjects | |
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Abstract | Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction. |
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AbstractList | Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction. |
Author | CHOI, G. T. Y CASU, M GIBBONS, W. A |
AuthorAffiliation | Department of Pharmaceutical Chemistry, School of Pharmacy, University of London, U.K |
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Snippet | Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and... |
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SubjectTerms | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Body Fluids - chemistry Cells - chemistry Cholesterol - analysis Choline - analysis Chromatography, Ion Exchange Ethanolamine Ethanolamines - analysis Fatty Acids, Nonesterified - analysis Fatty Acids, Unsaturated - analysis Fundamental and applied biological sciences. Psychology Glycerides - analysis Hydrogen-Ion Concentration Lipids Magnetic Resonance Spectroscopy Miscellaneous Other biological molecules Phospholipids - analysis Rats Sphingolipids - analysis |
Title | N.m.r. lipid profiles of cells, tissues and body fluids : neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions |
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