N.m.r. lipid profiles of cells, tissues and body fluids : neutral, non-acidic and acidic phospholipid analysis of Bond Elut chromatographic fractions

• Published in: Biochemical journal Vol. 290; no. 3; pp. 717 - 721
• Main Authors: CHOI, G. T. Y, CASU, M, GIBBONS, W. A
• Format: Journal Article
• Language: English
• Published: Colchester Portland Press 15.03.1993
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Body Fluids - chemistry; Cells - chemistry; Cholesterol - analysis; Choline - analysis; Chromatography, Ion Exchange; Ethanolamine; Ethanolamines - analysis; Fatty Acids, Nonesterified - analysis; Fatty Acids, Unsaturated - analysis; Fundamental and applied biological sciences. Psychology; Glycerides - analysis; Hydrogen-Ion Concentration; Lipids; Magnetic Resonance Spectroscopy; Miscellaneous; Other biological molecules; Phospholipids - analysis; Rats; Sphingolipids - analysis; Biological fluid; Tissue; Analytical method; Lipids; Qualitative analysis; NMR spectrometry; Cell; Quantitative analysis; Ion exchange chromatography
• ISSN: 0264-6021; 1470-8728
• DOI: 10.1042/bj2900717

Although the advantages of in vitro n.m.r. analysis of cellular lipids have been documented, this purely n.m.r. approach also has drawbacks. Rapid and quantitative separation of total lipids into neutral lipids, non-esterified fatty acids, non-acidic phospholipids and acidic phospholipids using Bond Elut ion-exchange columns as demonstrated here permitted a more quantitative and complete n.m.r. analysis of glycerides, cholesterol, saturated and unsaturated sphingolipids and ether lipids, as well as of diacylcholine and ethanolamine lipids. Acidic lipids were also analysed. The fatty acid compositions of the intact lipids in each of the four Bond Elut fractions were determined from the n.m.r. spectrum of each fraction.