Characteristics of the MicroRNA Expression Profile of Exosomes Released by Vero Cells Infected with Porcine Epidemic Diarrhea Virus

Exosomes are nanoscale vesicles actively secreted by a variety of cells. They contain regulated microRNA (miRNA), allowing them to function in intercellular communication. In the present study, the role of exosomal miRNAs in porcine epidemic diarrhea virus (PEDV) infection was investigated using exo...

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Published inViruses Vol. 14; no. 4; p. 806
Main Authors Yin, Lei, Shen, Xuehuai, Yin, Dongdong, Wang, Jieru, Zhao, Ruihong, Dai, Yin, Pan, Xiaocheng
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 13.04.2022
MDPI
Subjects
Animals
Chlorocebus aethiops
Diarrhea
Drug development
Epidemics
exosome
Exosomes
Forkhead protein
Genes
Hepatitis
host–pathogen interactions
Infections
Libraries
MAP kinase
MicroRNAs
MicroRNAs - genetics
miRNA
Nanoparticles
Pathogenesis
porcine epidemic diarrhea virus
Porcine epidemic diarrhea virus - genetics
Signal Transduction
Software
Swine
Transmissible gastroenteritis
Transmission electron microscopy
Variance analysis
Vero Cells
Viruses
United States > US
China
microRNAs
host–pathogen interactions
porcine epidemic diarrhea virus
exosome
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Summary:Exosomes are nanoscale vesicles actively secreted by a variety of cells. They contain regulated microRNA (miRNA), allowing them to function in intercellular communication. In the present study, the role of exosomal miRNAs in porcine epidemic diarrhea virus (PEDV) infection was investigated using exosomes isolated from Vero cells infected with PEDV. The results of transmission electron microscopy observation showed that the exosomes are spherical in shape, uniform in size, and negatively stained in the membrane. Nanoparticle tracking analysis showed that the average exosome particle size is 130.5 nm. The results of miRNA sequencing showed that, compared with the control group, a total of 115 miRNAs are abnormally expressed in the exosomes of infected cells. Of these, 80 miRNAs are significantly upregulated and 35 miRNAs are significantly downregulated. Functional annotation analysis showed that the differentially expressed miRNAs are associated with PEDV infection through interaction with the cAMP, Hippo, TGF-beta, HIF-1, FoxO, MAPK, and Ras signaling pathways. Thus, our findings provide important information about the effects of PEDV infection on exosomal miRNA expression and will aid the search for potential anti-PEDV drug candidates.
Bibliography:These authors contributed equally to this work.
ISSN:1999-4915
1999-4915
DOI:10.3390/v14040806