Antimicrobial potential of a hydrolyzed protein extract of the microalgae Nannochloropsis sp
Microalgae are photosynthetic microorganisms widely used at the industrial level because of their high content of compounds with bioactive properties such as antimicrobial peptides. These compounds have a wide range of action mechanisms, do not produce the resistance of conventional antibiotics and...
Saved in:
Published in | Dyna (Medellín, Colombia) Vol. 86; no. 211; pp. 192 - 198 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Universidad Nacional de Colombia
01.10.2019
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Microalgae are photosynthetic microorganisms widely used at the industrial level because of their high content of compounds with bioactive properties such as antimicrobial peptides. These compounds have a wide range of action mechanisms, do not produce the resistance of conventional antibiotics and could be effective against bacteria, fungi, viruses, parasites, and even tumor cells. In this work, by mechanical cell lysis treatment, a protein extract of dry biomass of Nannochloropsis sp was obtained, subsequently, it was hydrolyzed with an enzyme complex of proteases, papain, and pancreatin, for 6 hours, at 44 ° C and pH 7. Finally, the antimicrobial activity of the fraction of hydrolyzed extract with a size less than 3 kDa was evaluated against Staphylococcus aureus, Escherichia coli, and Candida albicans, obtaining a percentage of growth inhibition of 93.1%, 96.6%, and 14.4% respectively, which represents a peptic extract with bacteriostatic and fungistatic properties. |
---|---|
ISSN: | 0012-7353 2346-2183 |
DOI: | 10.15446/dyna.v86n211.78865 |