Developmental expression of neural cell adhesion molecules of oligodendrocytes in vivo and in culture

Previously, we have shown that oligodendrocyte adhesion molecules are related to the 120,000-Mr neural cell adhesion molecule (NCAM-120). In this report, we present further evidence that the oligodendrocyte adhesion molecule is NCAM-120. Studies on the expression of NCAM-120 and other molecular form...

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Bibliographic Details
Published inJournal of neurochemistry Vol. 50; no. 6; p. 1830
Main Authors Bhat, S, Silberberg, D H
Format Journal Article
LanguageEnglish
Published England 01.06.1988
Subjects
Aging - metabolism
Animals
Antigens, Surface - metabolism
Brain - cytology
Brain - growth & development
Brain - metabolism
Calcium - pharmacology
Calcium-Binding Proteins
Cell Adhesion - drug effects
Cell Adhesion Molecules
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Immunoassay
Neuroglia - metabolism
Oligodendroglia - metabolism
Rats
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Summary:Previously, we have shown that oligodendrocyte adhesion molecules are related to the 120,000-Mr neural cell adhesion molecule (NCAM-120). In this report, we present further evidence that the oligodendrocyte adhesion molecule is NCAM-120. Studies on the expression of NCAM-120 and other molecular forms of NCAM in vivo in rat brain, in vitro in primary mixed cultures, and in cultures enriched for oligodendrocytes are described. Western blot analysis of rat brain using anti-NCAM showed that NCAM-120 first appears at postnatal day 7 and increases in quantity thereafter, coincident with the development of oligodendrocytes in vivo and comparable to the expression of myelin basic protein. Purified oligodendrocytes from 4-week-old rat brains expressed only NCAM-120. Quantitation of various forms of NCAMs in rat brain showed marked age-related differences in the expression of three molecular forms of NCAM. Immunofluorescence analysis showed that oligodendrocytes, at all ages tested, expressed NCAM, but in older oligodendrocytes, the intensity of staining was less. Western blot analysis of oligodendrocyte-enriched cultures showed that from day 1 after isolation (12 days of age) through day 7 after isolation (18 days of age) only NCAM-120 is seen. A possible role for NCAM in myelination and remyelination is discussed.
ISSN:0022-3042
DOI:10.1111/j.1471-4159.1988.tb02485.x