Backscatter multiple wavelength digital holography for color micro-particle imaging

• Published in: Applied optics. Optical technology and biomedical optics Vol. 61; no. 5; p. B83
• Main Authors: Giri, Ramesh, Berg, Matthew J
• Format: Journal Article
• Language: English
• Published: United States 10.02.2022
• ISSN: 2155-3165
• DOI: 10.1364/AO.441509

This work applies digital holography to image stationary micro-particles in color. The approach involves a Michelson interferometer to mix reference light with the weak intensity light backscattered from a distribution of particles. To enable color images, three wavelengths are used, 430, 532, and 633 nm, as primary light sources. Three separate backscattered holograms are recorded simultaneously, one for each wavelength, which are resolved without spectral cross talk using a three-CMOS prism sensor. Fresnel diffraction theory is used to render monochrome images from each hologram. The images are then combined via additive color mixing with red, green, and blue as the primary colors. The result is a color image similar in appearance to that obtained with a conventional microscope in white-light epi-illumination mode. A variety of colored polyethylene micro-spheres and nonspherical dust particles demonstrate the feasibility of the approach and illustrate the effect of simple speckle-noise suppression and white balance methods. Finally, a chromaticity analysis is applied that is capable of differentiating particles of different colors in a quantitative and objective manner.