SERS-based detection of the antibiotic ceftriaxone in spiked fresh plasma and microdialysate matrix by using silver-functionalized silicon nanowire substrates

• Published in: Talanta (Oxford) Vol. 271; p. 125697
• Main Authors: Liu, Chen, Franceschini, Célia, Weber, Susanne, Dib, Tony, Liu, Poting, Wu, Long, Farnesi, Edoardo, Zhang, Wen-shu, Sivakov, Vladimir, Luppa, Peter B., Popp, Jürgen, Cialla-May, Dana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2024
• Subjects: Ceftriaxone; Fresh plasma; Microdialysate; Nanoparticles; Quantitative analysis; SERS; Silicon nanowires; Silver; TDM; Nanoparticles; Silver; SERS; Fresh plasma; Microdialysate; Ceftriaxone; TDM; Silicon nanowires; Quantitative analysis
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2024.125697

Therapeutic drug monitoring (TDM) is an important tool in precision medicine as it allows estimating pharmacodynamic and pharmacokinetic effects of drugs in clinical settings. An accurate, fast and real-time determination of the drug concentrations in patients ensures fast decision-making processes at the bedside to optimize the clinical treatment. Surface-enhanced Raman spectroscopy (SERS), which is based on the application of metallic nanostructured substrates to amplify the inherent weak Raman signal, is a promising technique in medical research due to its molecular specificity and trace sensitivity accompanied with short detection times. Therefore, we developed a SERS-based detection scheme using silicon nanowires decorated with silver nanoparticles, fabricated by means of top-down etching combined with chemical deposition, to detect the antibiotic ceftriaxone (CRO) in spiked fresh plasma and microdialysis samples. We successfully detected CRO in both matrices with an LOD of 94 μM in protein-depleted fresh plasma and 1.4 μM in microdialysate. [Display omitted] •Ag@SiNWs SERS substrates enabled quantification of ceftriaxone in blood-derived fluids.•For fresh plasma, competition for free binding sites at the sensor surface can be avoided by protein precipitation.•Use of microdialysates reduces interfering background signals in ceftriaxone detection.•Direct SERS in blood-derived, protein-depleted fluids has a great potential for TDM.