Physicochemical Investigation of Biosynthesis of a Protein Coating on Glass That Promotes Mammalian Cell Growth Using Lactobacillus rhamnosus GG Bacteria

Glass surfaces, although the first to be used for culturing ex vivo adherent cells, are not the perfect substrates for this purpose. Today, plastics dominate these applications, but in light of the global trend to reduce the use of synthetic polymers, it is reasonable to consider a return to glass v...

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Bibliographic Details
Published inCoatings (Basel) Vol. 11; no. 11; p. 1410
Main Authors Kaminski, Kamil, Syrek, Karolina, Grudzień, Joanna, Obloza, Magdalena, Adamczyk, Monika, Sulka, Grzegorz D.
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2021
Subjects
Antibiotics
Bacteria
Biosynthesis
Cell culture
Cell growth
Coatings
Collagen
Ethanol
Glass substrates
Gold
Infrared spectra
Laboratories
Lactobacilli
Mammals
Microorganisms
Nanoparticles
Optical microscopy
Penicillin
Polymers
Proteins
Substrates
Thermal degradation
Ultraviolet reflection
Ultraviolet spectra
United States > US
Japan
Germany
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Summary:Glass surfaces, although the first to be used for culturing ex vivo adherent cells, are not the perfect substrates for this purpose. Today, plastics dominate these applications, but in light of the global trend to reduce the use of synthetic polymers, it is reasonable to consider a return to glass vessels with coatings for these purposes. The ideal surface for cell growth is one that simulates the composition and structure of the mainly protein-based intercellular matrix. The work presented here shows a new idea of preparing porous protein coatings on glass using biosynthesis. The process utilizes the colonization of the gold nanoparticle-coated glass surface with Lactobacillus rhamnosus GG bacteria, followed by permeabilization (using ethanol) of their membrane and partial thermal degradation (at 160 °C in vacuum) of the surface-bound protein components of these microorganisms. It results in a development of coating on the glass that promotes mammalian cell growth, which has been preliminary confirmed using Vero cells. Subsequent steps in the formation of coating components were documented by reflectance ultraviolet and visible spectra and infrared spectroscopy. The presence of microorganisms and mammalian cells was confirmed using scanning electron and optical microscopy and crystalline violet staining.
ISSN:2079-6412
2079-6412
DOI:10.3390/coatings11111410