Label-free multimodal microscopy using a single light source and detector for biological imaging

Multimodal nonlinear microscopy has been widely applied in biology and medicine due to its relatively deep penetration into tissue and its label-free manner. However, current multimodal systems require the use of multiple sources and detectors, leading to bulky, complex, and expensive systems. In th...

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Published inOptics letters Vol. 46; no. 4; p. 892
Main Authors Kang, Juehyung, Kang, Ungyo, Nam, Hyeong Soo, Kim, Wooseop, Kim, Hyun Jung, Kim, Ryeong Hyeon, Kim, Jin Won, Yoo, Hongki
Format Journal Article
LanguageEnglish
Published United States 15.02.2021
Subjects
Animals
Coronary Vessels - diagnostic imaging
Light
Microscopy - instrumentation
Microscopy - methods
Multimodal Imaging - instrumentation
Multimodal Imaging - methods
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
Swine
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Summary:Multimodal nonlinear microscopy has been widely applied in biology and medicine due to its relatively deep penetration into tissue and its label-free manner. However, current multimodal systems require the use of multiple sources and detectors, leading to bulky, complex, and expensive systems. In this Letter, we present a novel method of using a single light source and detector for nonlinear multimodal imaging of biological samples. Using a photonic crystal fiber, a pulse picker, and multimode fibers, our developed system successfully acquired multimodal images of swine coronary arteries, including two-photon excitation fluorescence, second-harmonic generation, coherent anti-Stokes Raman scattering, and backreflection. The developed system could be a valuable tool for various biomedical applications.
ISSN:1539-4794
DOI:10.1364/OL.415938