Association of aberrant brain network dynamics with gut microbial composition uncovers disrupted brain-gut-microbiome interactions in irritable bowel syndrome: Preliminary findings

• Published in: European journal of neurology Vol. 30; no. 11; pp. 3529 - 3539
• Main Authors: Yang, Lin, Liu, Guangyao, Li, Shan, Yao, Chaofan, Zhao, Ziyang, Chen, Nan, Zhang, Pengfei, Shang, Yingying, Wang, Yin, Zhang, Dekui, Tian, Xiaozhu, Zhang, Jing, Yao, Zhijun, Hu, Bin
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.11.2023
• Subjects: Abnormalities; Brain; Brain mapping; Composition; Digestive system; Feces; Functional magnetic resonance imaging; Gene sequencing; Intestinal microflora; Intestine; Irritable bowel syndrome; Magnetic resonance imaging; Microbiomes; Microbiota; Microorganisms; Neural networks; Neuroimaging; Pathogenesis; rRNA 16S; dynamic functional connectivity; brain-gut-microbiome axis; gut microbiome; irritable bowel syndrome; resting-state functional MRI
• ISSN: 1351-5101; 1468-1331
• DOI: 10.1111/ene.15776

Growing evidence suggests that abnormalities in brain-gut-microbiome (BGM) interactions are involved in the pathogenesis of irritable bowel syndrome (IBS). Our study aimed to explore alterations in dynamic functional connectivity (DFC), the gut microbiome and the bidirectional interaction in the BGM. Resting-state functional magnetic resonance imaging (rs-fMRI), fecal samples and clinical chacteristics were collected from 33 IBS patients and 32 healthy controls. We performed a systematic DFC analysis on rs-fMRI. The gut microbiome was analyzed by 16S rRNA gene sequencing. Associations between DFC characteristics and microbial alterations were explored. In the DFC analysis, four dynamic functional states were identified. IBS patients exhibited increased mean dwell and fraction time in State 4, and reduced transitions from State 3 to State 1. Aberrant temporal properties in State 4 were only evident when choosing a short window (36 s or 44 s). Decreased functional connectivity (FC) variability was found in State 1 and State 3 in IBS patients, two of which (independent component [IC]51-IC91, IC46-IC11) showed significant correlations with clinical characteristics. Additionally, we identified nine significantly differential abundances in microbial composition. We also found that IBS-related microbiota were associated with aberrant FC variability, although these exploratory results were obtained at an uncorrected threshold of significance. Although future studies are needed to confirm our results, the findings not only provide a new insight into the dysconnectivity hypothesis in IBS from a dynamic perspective, but also establish a possible link between DFC and the gut microbiome, which lays the foundation for future research on disrupted BGM interactions.