Separation and Enrichment of Baicalin in SBG by Solvent Sublation and Its Determination by HPLC and Spectroscopy

• Published in: Analytical letters Vol. 38; no. 2; pp. 257 - 270
• Main Authors: Dong, Hui-Ru, Bi, Peng-Yu, Wang, Shi-Hui
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 01.01.2005; Taylor & Francis
• Subjects: Analytical chemistry; Baicalin; Chemistry; Chromatographic methods and physical methods associated with chromatography; determination; enrichment; Exact sciences and technology; HPLC; Other chromatographic methods; Scutellaria Baicalensis Georgi; separation; Solvent sublation; Spectrometric and optical methods; spectroscopy; Fourier transform spectroscopy; HPLC chromatography; NMR spectrometry; determination; Nitrogen; Sodium chloride; Scutellaria Baicalensis Georgi; Chemical enrichment; Infrared spectrometry; Organic solvent; enrichment; Precipitation; Efficiency; separation; spectroscopy; Solvent sublation; Baicalin; HPLC
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1081/AL-200045147

An effective method for separating and enriching Baicalin from Scutellaria Baicalensis Georgi (SBG) by solvent sublation and, subsequently, identifying it by HPLC and spectroscopy is described in this paper. The effects of the organic solvent, nitrogen flow rate, solution pH, sublation time, and electrolyte sodium chloride on the sublation efficiency were investigated in detail, and the optimal conditions of SBG solvent sublation were determined. The floated product of SBG under the optimal conditions was positively identified by HPLC, UV, FTIR, and 1 H-NMR. Solvent sublation was compared with the traditional precipitation method and was found to generate a significantly better product. The results show that the proposed method is simple, quick, and selective.