Use of monoclonal antibodies to detect Mn(II)-peroxidase in birch wood degraded by Phanerochaete chrysosporium

• Published in: Applied microbiology and biotechnology Vol. 35; no. 5; pp. 674 - 680
• Main Authors: Daniel, G, Jellison, J, Goodell, B, Paszczynski, A, Crawford, R
• Format: Journal Article
• Language: English
• Published: 01.08.1991
• Subjects: Betula; biodegradation; cell walls; characterization; decay fungi; decayed wood; immunocytochemistry; manganese; monoclonal antibodies; peroxidases; Phanerochaete chrysosporium; purification
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/BF00169636

A monoclonal antibody (Mab) produced to purified Mn(II)-peroxidase was visualized on and within cell comers of birch wood degraded by Phanerochaete chrysosporium using colloidal gold immuno-transmission electron microscopy techniques. Labelling of the fungal cell membrane and cell wall was also observed. The same Mab was used to visualize the penetration of extracellular fungal metabolite extracts, infiltrated into previously decayed wood. Binding of antibodies to the lignin-rich cell corner region of the middle lamella in wood decayed by P. chrysosporium was observed in sectioned wood blocks and in wood infiltrated with crude extracellular extracts from P. chrysosporium liquid cultures. When a control monoclonal antiserum, produced to extracellular metabolites of Postia (Poria) placenta and cross-reactive with fungal cellulase, was used in labelling, the cellulose rich region of the wood cell walls were labelled. Labelling in the middle lamella cell corners was only noted in what has been described as non-or poorly lignified cell comer regions.