Detection of HBsAg in a clone derived from the PLC/PRF/5 human hepatoma cell line

A total of 28 clones were established from the PLC/PRF/5 hepatoma cell line by a plating procedure. All clones were found to secrete HBsAg into the supernatant culture fluids. Of these, one clone (No. 23) free of detectable mycoplasma contamination and showing smooth epithelial morphology was select...

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Bibliographic Details
Published inArchives of virology Vol. 68; no. 3-4; p. 157
Main Authors Wen, Y M, Copeland, J A, Mann, G F, Howard, C R, Zuckerman, A J
Format Journal Article
LanguageEnglish
Published Austria 01.01.1981
Subjects
Carcinoma, Hepatocellular - immunology
Carcinoma, Hepatocellular - metabolism
Cell Line
Clone Cells - immunology
Fluorescent Antibody Technique
Hepatitis B Surface Antigens - immunology
Hepatitis B Surface Antigens - metabolism
Humans
Kinetics
Liver Neoplasms - immunology
Liver Neoplasms - metabolism
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Summary:A total of 28 clones were established from the PLC/PRF/5 hepatoma cell line by a plating procedure. All clones were found to secrete HBsAg into the supernatant culture fluids. Of these, one clone (No. 23) free of detectable mycoplasma contamination and showing smooth epithelial morphology was selected for further study. Maximum accumulation of HBsAg occurred 9 days after sub-culture and intracellular antigen was detected by indirect immunofluorescence both in the cytoplasm and at the plasma membrane. Granules and perinuclear staining reactions were also observed in clone 23 cells and these findings are compared to the previously published properties of the parental PLC/PRF/5 cell line.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01314568