Nematicidal activity of paucimannose-type glycoconjugates from acacia honey

Natural honey contains glycoconjugates as minor components. We characterized acacia honey glycoconjugates with molecular masses in the range of 2–5 kDa. The glycoconjugates were separated by RP-HPLC into three peaks (termed RP-2-5 k-I, RP-2-5 k-II, and RP-2-5 k-III) which demonstrated paralyzing eff...

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Bibliographic Details
Published inExperimental parasitology Vol. 259; p. 108707
Main Authors Bilal, Bushra, Azim, M. Kamran
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.2024
Subjects
Antinematodal
Apis mellifera
Bee products
Honey glycoconjugates
paucimannose
Apis mellifera
Bee products
Honey glycoconjugates
paucimannose
Antinematodal
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Summary:Natural honey contains glycoconjugates as minor components. We characterized acacia honey glycoconjugates with molecular masses in the range of 2–5 kDa. The glycoconjugates were separated by RP-HPLC into three peaks (termed RP-2-5 k-I, RP-2-5 k-II, and RP-2-5 k-III) which demonstrated paralyzing effects on the model nematode C. elegans (ED50 of 50 ng glycoconjugates/μL). To examine molecular mechanisms underlying the nematicidal effects of honey glycoconjugates, expressional analyses of genes that are essential for the growth, development, reproduction, and movement of C. elegans were carried out. Quantitative PCR-based assays showed that these molecules moderately regulate the expression of genes involved in the citric acid cycle (mdh-1 and idhg-1) and cytoskeleton (act-1 and act-2). MALDI-ToF-MS/MS analysis of RP-HPLC peaks revealed the presence of paucimannose-like N-glycans which are known to play important roles in invertebrates e.g., worms and flies. These findings provided novel information regarding the structure and nematicidal function of honey glycoconjugates. [Display omitted] •Glycoconjugates of honey showed C. elegans paralyzing activity.•Exposure of honey glycoconjugates caused altered regulation of C. elegans genes transcripts involved in the citric acid cycle, cytoskeleton, body morphogenesis, embryo development.•Characterization of nematicidal glycoconjugates from honey by mass spectrometry revealed paucimannose like N-Glycans which are known to play important roles in survival of invertebrates e.g., worms and flies.
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ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2024.108707