Small-angle X-ray scattering probe of intermolecular interaction in red blood cells

With high concentrations of hemoglobin （Hb） in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentration Hb in solution and red blood cells were analyzed by small-angle X-ray scattering. C...

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Published inChinese physics C Vol. 39; no. 3; pp. 83 - 86
Main Author 刘广峰 王文佳 徐建华 董宇辉
Format Journal Article
LanguageEnglish
Published 01.03.2015
Subjects
Agglomeration
Aggregates
Hemoglobin
Mathematical analysis
Physiological effects
Red blood cells
Saline
Small angle X ray scattering
分子间相互作用
小角X射线散射
小角度X射线散射
探测
红血细胞
细胞浓度
自相互作用
蛋白分子
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Summary:With high concentrations of hemoglobin （Hb） in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentration Hb in solution and red blood cells were analyzed by small-angle X-ray scattering. Calculation of the effective structure factor indicates that the interaction of Hb molecules is the same when they are crowded together in both the cell and physiological saline. The Hb molecules stay individual without the formation of aggregates and clusters in cells.
Bibliography:With high concentrations of hemoglobin （Hb） in red blood cells, self-interactions among these molecules could increase the propensities of their polymerization and aggregation. In the present work, high concentration Hb in solution and red blood cells were analyzed by small-angle X-ray scattering. Calculation of the effective structure factor indicates that the interaction of Hb molecules is the same when they are crowded together in both the cell and physiological saline. The Hb molecules stay individual without the formation of aggregates and clusters in cells.
11-5641/O4
small-angle X-ray scattering, protein-protein interaction, hemoglobin
LIU Guan-Fen WANG We-Ji XU Jia-Hua DONG Yu-Hui（ Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China）
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1674-1137
0254-3052
DOI:10.1088/1674-1137/39/3/038001