Effects of early-life stress followed by access to stevia or sucralose during adolescence on weight gain, glycemia, and anxiety-related behaviors in male and female rats

• Published in: Physiology & behavior Vol. 280; p. 114529
• Main Authors: Rico, Javier Leonardo, Aya-Ramos, Laura, Dueñas, Zulma
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2024
• Subjects: Anxiety; Blood glucose; Maternal separation; Open-field; Stevia; Sucralose; Blood glucose; Open-field; Sucralose; NNS; Maternal separation; PND; OF; Anxiety; Stevia
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2024.114529

•The effects of early stress and non-nutritive sweeteners (NNS) intake are unknown.•Early-stressed rats had high blood glucose levels during NNS consumption.•Early maternal separation stress increased anxiety and locomotion in female rats.•NNS consumption reduced anxiety in maternally separated female but not male rats.•Stevia and sucralose seem had an anxiolytic-like effect in early stressed females. Early-life stress and subsequent high-calorie diets during adolescence are known to be risk factors for developing metabolic and psychological disorders. Although non-nutritive sweeteners such as stevia and sucralose have been a useful alternative to reduce sugar consumption, the effects of prolonged consumption of these sweeteners on metabolism and behavior in adolescents remain unclear. Here, we evaluated the effects of early-stress followed by access to stevia or sucralose during adolescence on weight gain, glycemia, and anxiety-related behaviors in male and female rats. During postnatal days (PNDs) 1–21, pups were separated twice a day, for 180 min each time, from their dam nest while non-separated pups served as controls. The pups were weaned, separated by sex and randomly distributed into the stevia, sucralose and water conditions. During PNDs 26–50, two bottles containing water and stevia or sucralose were placed in the animal home-cages, and body weight and blood glucose measures were scored. On PNDs 50 and 51, behavioral measures were obtained in the open-field test. Results showed that male rats consuming stevia reduced body weight gain, blood glucose and increased locomotion. Early-stress led to low blood glucose and alterations in anxiety and locomotion-related behaviors in a sex-dependent manner. Moreover, sucralose access during adolescence reversed the effects of early-stress on anxiety-related behaviors in female rats. The results suggest that the consumption of stevia and sucralose could be an alternative for the replacement of sugar-sweetened beverages, especially in adolescents who have had adverse early-life experiences. [Display omitted]