Whole Genome Sequencing and Comparisons of Different Chinese Rabies Virus Lineages Including the First Complete Genome of an Arctic-like Strain in China

Objective To learn the rabies genome molecular characteristics and compare the difference of China rabies lineages. Methods The complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes （total 43）, Arctic and Arctic-like...

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Published inBiomedical and environmental sciences Vol. 29; no. 5; pp. 340 - 346
Main Authors LI, Hao, GUO, Zhen Yang, ZHANG, Jian, TAO, Xiao Yan, ZHU, Wu Yang, TANG, Qing, LIU, Hong Tu
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.05.2016
Key Laboratory for Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention, Beijing 102206, China
Subjects
Animals
Brain - virology
Cattle
China
Dogs
Genome
Genome, Viral
Humans
Lineage
Phylogeny
Rabies - virology
Rabies virus
Rabies virus - genetics
Sequence Analysis, DNA
Viral Proteins - genetics
中国
全基因组测序
分子特征
北极
毒株
狂犬病病毒
系统进化树
谱系
Lineage
Genome
Rabies virus
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Summary:Objective To learn the rabies genome molecular characteristics and compare the difference of China rabies lineages. Methods The complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes （total 43）, Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5. Results First Arctic-like rabies genome in China （CO, H1202D） was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V （11908nt） is obviously shorter than other lineages＇ （11923-11925nt） for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%. CQH1202D （China IV lineage） has close relationship with strains from South Korea and they share about 95% genome similarities. Conclusion The molecular characteristics of 6 different China rabies lineages were compared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
Bibliography:Rabies virus; Genome; Lineage
11-2816/Q
Objective To learn the rabies genome molecular characteristics and compare the difference of China rabies lineages. Methods The complete genomes of 12 strains from different China rabies lineages were amplified and sequenced, and all the China street strain genomes （total 43）, Arctic and Arctic-like genomes were aligned using ClustalX2, the genome homologies were analyzed using MegAlign software, and the phylogenetic trees were constructed by MEGA 5. Results First Arctic-like rabies genome in China （CO, H1202D） was reported, and we supplemented the rabies genome data of China, ensuring at least one genome was available in each China lineage. The genome size of China V （11908nt） is obviously shorter than other lineages＇ （11923-11925nt） for the difference of N-P non-coding regions. Among different lineages, the genome homologies are almost under 90%. CQH1202D （China IV lineage） has close relationship with strains from South Korea and they share about 95% genome similarities. Conclusion The molecular characteristics of 6 different China rabies lineages were compared and analyzed from genome level, which benefits for continued comprehensive rabies surveillance, rabies prevention and control in China.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0895-3988
2214-0190
DOI:10.3967/bes2016.044