Multi-omics analysis reveals the microbial interactions of S. cerevisiae and L. plantarum on Suanyu, Chinese traditional fermented fish

• Published in: Food research international Vol. 174; no. Pt 1; p. 113525
• Main Authors: Wenkang, Hu, Jingui, Liu, Wei, Zhang, Jiangli, Wu, Zhengbin, Yang, Furong, Zhang, Xuefeng, Zeng
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.12.2023
• Subjects: Animals; Fermentation; Fermented Foods - microbiology; L. plantarum; Lactobacillus plantarum - metabolism; Metabolomics; Microbial interaction; Microbial Interactions; Multiomics; S. cerevisiae; Saccharomyces cerevisiae - metabolism; Seafood - microbiology; Suanyu; Transcriptomics; Microbial interaction; Metabolomics; L. plantarum; Suanyu; Transcriptomics; S. cerevisiae
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2023.113525

[Display omitted] •Explored the mechanism of microbial interactions through physicochemical, Metabolomics, transcriptome analysis.•Co-fermentation can affect different metabolic pathways of both micrograms.•Even as the dominant strain, some life activities of S. cerevisiae were still inhibited. S. cerevisiae and L. plantarum play important roles in Suanyu fermentation. This study investigated the interaction between S. cerevisiae and L. plantarum during fermentation and its impact on metabolic pathways. Co-culturing S. cerevisiae and L. plantarum increased pH to 5.72, reduced TVB-N to 9.47 mg/mL, and achieved high utilization rates of sugars (98.9%) and proteins (73.7%). During microbial interactions, S. cerevisiae and L. plantarum produced antibiotics, including phenyllactate and Gentamicin C1a, inhibiting the growth of each other. S. cerevisiae used S-adenosyl-l-methionine to counteract acid production of L. plantarum, establishing dominance in Suanyu fermentation. Microbial interactions influenced carbohydrate and energy metabolism pathways, such as nicotinate and nicotinamide metabolism and purine metabolism. S. cerevisiae significantly impacted gene expression in protein synthesis and cell growth pathways, including ribosome, SNARE interactions, basal transcription factors, and MAPK signaling. These findings offer insights into microbial interactions and metabolic processes during Suanyu fermentation.