Characterization of pig-mink cell hybrids: assignment of the TK1 and UMPH2 genes to pig chromosome 12

By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or...

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Bibliographic Details
Published inMammalian genome Vol. 5; no. 12; p. 781
Main Authors Zhdanova, N S, Astakhova, N M, Kuznetsov, S B, Schuler, L, Serov, O L
Format Journal Article
LanguageEnglish
Published United States 01.12.1994
Subjects
5'-Nucleotidase - genetics
5'-Nucleotidase - metabolism
Animals
Cell Line
Chromosome Mapping - methods
Cloning, Molecular
Fibroblasts - cytology
Fibroblasts - enzymology
Hybrid Cells
Isoenzymes - genetics
Isoenzymes - metabolism
Lymphocytes - cytology
Lymphocytes - enzymology
Male
Mink
Mutagenesis, Insertional
Phosphates - metabolism
Swine
Thymidine Kinase - genetics
Thymidine Kinase - metabolism
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Summary:By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
ISSN:0938-8990
DOI:10.1007/BF00292013