Identification of hexapeptides that render C2 myoblasts the resistant to menadione-induced cell death

Menadione induced cell death in cultured C2 myoblasts. By screening synthetic peptide libraries composed of random sequence of hexapeptides, we identified the hexa-peptides pool of (Ala/Ile)-(Ile/Met)-Val-Ile-Asp- Met/Ser)-NH₂ that protected the myoblasts against menadione-induced cell death. Pre-in...

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Published inAnimal cells and systems Vol. 12; no. 1
Main Authors Hwang, S.H. (Ajou University, Suwon, Republic of Korea), Kim, M.J. (Ajou University, Suwon, Republic of Korea), Lim, J.A. (Ajou University, Suwon, Republic of Korea), Woo, J.H. (Ajou University, Suwon, Republic of Korea), Kim, H.S. (Ajou University, Suwon, Republic of Korea), E-mail: hsunkim@ajou.ac.kr
Format Journal Article
LanguageEnglish
Published 2008
Subjects
Bcl-2
C2 myolblasts
cell death
ESTRES
hexapeptides
menadione
OXIDACION
OXIDATION
OXYDATION
STRESS
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Summary:Menadione induced cell death in cultured C2 myoblasts. By screening synthetic peptide libraries composed of random sequence of hexapeptides, we identified the hexa-peptides pool of (Ala/Ile)-(Ile/Met)-Val-Ile-Asp- Met/Ser)-NH₂ that protected the myoblasts against menadione-induced cell death. Pre-incubation with the hexapeptide pool reduced the number of cells detached from culture dish substrate and increased the ratio of relative viability against menadione. In addition, the peptides strongly increased the expression of Bcl-2, an anti-apoptotic protein. These results suggest that the hexapeptides might enhance the resistance to cell death against menadione by increasing the expression of Bcl-2.
Bibliography:2009001097
L01
ISSN:1976-8354
2151-2485
DOI:10.1080/19768354.2008.9647151