Anthelmintic efficacy of an organic fraction from Guazuma ulmifolia leaves and evaluation of reactive oxidative stress on Haemonchus contortus

• Published in: Experimental parasitology Vol. 261; p. 108768
• Main Authors: Reséndiz-González, Guillermo, Olmedo-Juárez, Agustín, González-Garduño, Roberto, Cortes Morales, Jorge Alberto, Cortazar, Manasés González, Sánchez-Mendoza, Ana Elvia, López-Arellano, María Eugenia, Mercado-Márquez, Crisóforo, Lara-Bueno, Alejandro, Higuera-Piedrahita, Rosa Isabel
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2024
• Subjects: Anthelmintic; Glutathione; Guazuma ulmifolia; Over expression; Guazuma ulmifolia; Anthelmintic; Over expression; Glutathione; anthelmintic; over expression; glutathione
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2024.108768

This study describes the anthelmintic efficacy of an organic fraction (EtOAc-F) from Guazuma ulmifolia leaves and the evaluation of its reactive oxidative stress on Haemonchus contortus. The first step was to assess the anthelmintic effect of EtOAc-F at 0.0, 3.5, 7.0 and 14 mg kg of body weight (BW) in gerbil's (Meriones unguiculatus) artificially infected with H. contortus infective larvae (L3). The second step was to evaluate the preliminary toxicity after oral administration of the EtOAc-F in gerbils. Finally, the third step was to determine the relative expression of biomarkers such as glutathione (GPx), catalase (CAT), and superoxide dismutase (SOD) against H. contortus L3 post-exposition to EtOAc-F. Additionally, the less-polar compounds of EtOAc-F were identified by gas mass spectrophotometry (GC-MS). The highest anthelmintic efficacy (97.34%) of the organic fraction was found in the gerbils treated with the 14 mg/kg of BW. Histopathological analysis did not reveal changes in tissues. The relative expression reflects overexpression of GPx (p<0.05, fold change: 14.35) and over expression of SOD (p≤0.05, fold change: 0.18) in H. contortus L3 exposed to 97.44 mg/mL of EtOAc-F compared with negative control. The GC-MS analysis revealed the presence of 4-hydroxybenzaldehyde (1), leucoanthocyanidin derivative (2), coniferyl alcohol (3), ferulic acid methyl ester acetate (4), 2,3,4-trimethoxycinnamic acid (5) and epiyangambin (6) as major compounds. According to these results, the EtOAc-F from G. ulmifolia leaves exhibit anthelmintic effect and increased the stress biomarkers on H. contortus. [Display omitted] •Ethyl acetate fraction reduces the larvae count in the in vivo gerbil test.•There were no histopathological lesions attributable to the treatments in gerbils.•Ethyl acetate fraction increases stress biomarkers on Haemonchus contortus larvae.