Molecular cloning of TaMATE2 homoeologues potentially related to aluminium tolerance in bread wheat (Triticum aestivum L.)

• Published in: Plant biology (Stuttgart, Germany) Vol. 20; no. 5; pp. 817 - 824
• Main Authors: Garcia-Oliveira, A L, Benito, C, Guedes-Pinto, H, Martins-Lopes, P
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.09.2018
• Subjects: Aluminum; Aluminum - toxicity; Bread; Chromosomes; Citric acid; Cloning; Cloning, Molecular; Cultivars; Efflux; Gene Expression Profiling; Genes, Plant - genetics; Genotype; Genotypes; Organic Cation Transport Proteins - genetics; Phylogeny; Plant Proteins - genetics; Plant Proteins - metabolism; Plant tissues; Pollution tolerance; Protein structure; Rye; Secondary structure; Transcription; Transmembrane domains; Triticum - genetics; Triticum - metabolism; Triticum aestivum; Wheat; Triticum aestivum L; homoeologues; candidate gene; TaMATE2; Al tolerance
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.12864

Recently, members of the MATE family have been implicated in aluminium (Al) tolerance by facilitating citrate efflux in plants. The aim of the present work was to perform a molecular characterisation of the MATE2 gene in bread wheat. Here, we cloned a member of the MATE gene family in bread wheat and named it TaMATE2, which showed the typical secondary structure of MATE-type transporters maintaining all the 12 transmembrane domains. Amplification in Chinese Spring nulli-tetrasomic and ditelosomic lines revealed that TaMATE2 is located on the long arm of homoeologous group 1 chromosomes. The transcript expression of TaMATE2 homoeologues in two diverse bread wheat genotypes, Barbela 7/72/92 (Al-tolerant) and Anahuac (Al-sensitive), suggested that TaMATE2 is expressed in both root and shoot tissues of bread wheat, but mainly confined to root rather than shoot tissues. A time-course analysis of TaMATE2 homoeologue transcript expression revealed the Al responsive expression of TaMATE2 in root apices of the Al-tolerant genotype, Barbela 7/72/92. Considering the high similarity of TaMATE2 together with similar Al responsive expression pattern as of ScFRDL2 from rye and OsFRDL2 from rice, it is likely that TaMATE2 also encodes a citrate transporter. Furthermore, the TaMATE2-D homoeologue appears to be near the previously reported locus (wPt0077) on chromosome 1D for Al tolerance. In conclusion, molecular cloning of TaMATE2 homoeologues, particularly TaMATE2-D, provides a plausible candidate for Al tolerance in bread wheat that can be used for the development of more Al-tolerant cultivars in this staple crop.